Anti-FKBP12 antibody [EP3887] (ab108420)
![Anti-FKBP12 antibody [EP3887] (ab108420)](https://www.abcam.com/ps/products/108/ab108420/Images/ab108420-297790-anti-fkbp12-antibody-ep3887-western-blot.jpg "Anti-FKBP12 antibody [EP3887] (ab108420)")
Key features and details
- Rabbit monoclonal [EP3887] to FKBP12
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FKBP12 antibody [EP3887]
See all FKBP12 primary antibodies -
Description
Rabbit monoclonal [EP3887] to FKBP12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HL-60, U937 and SH-SY5Y lysates; HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3887 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab108420 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108420 was shown to recognize FKBP12 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP12 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab108420 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)](https://www.abcam.com/ps/products/108/ab108420/Images/ab108420-121137-anti-fkbp12-antibody-ep3887-western-blot.jpg)
Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)All lanes : Anti-FKBP12 antibody [EP3887] (ab108420) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : HL-60 brain lysate
Lane 3 : U937 lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 12 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody [EP3887] (ab108420)](https://www.abcam.com/ps/products/108/ab108420/Images/ab108420-121136-anti-fkbp12-antibody-ep3887-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody [EP3887] (ab108420)Immunofluorescent staining of FKBP12 in HeLa cells using ab108420 at 1/50 dilution.
