Human FKBP12 ELISA kit (ab277418)
Key features and details
- Sensitivity: 0.81 ng/ml
- Range: 0.819 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FKBP12 ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.81 ng/ml -
Range
0.819 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 84.49 83% - 87% Plasma 74.91 71% - 87% Cell culture media 72.67 70% - 76% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human FKBP12 ELISA Kit (ab277418) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FKBP12 in serum (human FKBP12 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human FKBP12 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human FKBP12 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human FKBP12 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human FKBP12 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated Anti-Human FKBP12 Antibody 2 vials Anti-Human FKBP12 coated Microplate 1 unit Human FKBP12 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
Contains 1 PPIase FKBP-type domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- 12 kDa FK506-binding protein
- 12 kDa FKBP
- Calstabin 1
see all -
Database links
- Entrez Gene: 2280 Human
- Omim: 186945 Human
- SwissProt: P62942 Human
- Unigene: 471933 Human
- Unigene: 700839 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.