Anti-Cyclophilin A antibody (ab3563)
Key features and details
- Rabbit polyclonal to Cyclophilin A
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclophilin A antibody
See all Cyclophilin A primary antibodies -
Description
Rabbit polyclonal to Cyclophilin A -
Host species
Rabbit -
Specificity
Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African green monkey
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Immunogen
Other Immunogen Type corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cyclophilin A antibody (ab3563)
ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)ICC/IF image of ab3563 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3563, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Cyclophilin A antibody (ab3563)All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : NIH-3T3
Lysates/proteins at 25 µg per lane.
Observed band size: 18 kDa why is the actual band size different from the predicted?
