Anti-Cyclophilin A antibody (ab41684)
Key features and details
- Rabbit polyclonal to Cyclophilin A
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Cyclophilin A antibody
See all Cyclophilin A primary antibodies -
Description
Rabbit polyclonal to Cyclophilin A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB HumanRecombinant fragment
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Cyclophilin A.
Read Abcam's proprietary immunogen policy (Peptide available as ab42406.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanRecombinant fragmentAll applications MouseCowDogPigMonkeyGorillaChinese hamsterOrangutanApplication Abreviews Notes WB (3) Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).ICC/IF (1) Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5478 Human
- Entrez Gene: 268373 Mouse
- Entrez Gene: 100360977 Rat
- Entrez Gene: 100362969 Rat
- Entrez Gene: 25518 Rat
- Omim: 123840 Human
- SwissProt: P62937 Human
- SwissProt: P17742 Mouse
see all -
Alternative names
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
see all
Images
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Western blot - Anti-Cyclophilin A antibody (ab41684)All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab41684)ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab41684)This image is courtesy of an abreview submitted by Alina Macovei.Immunocytochemistry/ Immunofluorescence analysis of HepaRG cells labeling Cyclophilin A with ab41684 at 1μg/ml. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS. The cells were blocked with 1% milk for 20 minutes at room temperature. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/400 dilution.
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Western blot - Anti-Cyclophilin A antibody (ab41684)ab88202 is a GST-tagged recombinant protein with an expected molecular weight of 50kDa.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab41684 has been referenced in 37 publications.
- Langer HT et al. Nerve damage induced skeletal muscle atrophy is associated with increased accumulation of intramuscular glucose and polyol pathway intermediates. Sci Rep 10:1908 (2020). PubMed: 32024865
- Hu H et al. Cyclophilin A inhibits trophoblast migration and invasion in vitro and vivo through p38/ERK/JNK pathways and causes features of preeclampsia in mice. Life Sci 261:118351 (2020). PubMed: 32858039
- Tsachaki M et al. Impact of 17ß-HSD12, the 3-ketoacyl-CoA reductase of long-chain fatty acid synthesis, on breast cancer cell proliferation and migration. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31302749
- Pahk K et al. SP-8356, a Novel Inhibitor of CD147-Cyclophilin A Interactions, Reduces Plaque Progression and Stabilizes Vulnerable Plaques in apoE-Deficient Mice. Int J Mol Sci 21:N/A (2019). PubMed: 31877775
- Dhanda AS et al. Listeria monocytogenes hijacks CD147 to ensure proper membrane protrusion formation and efficient bacterial dissemination. Cell Mol Life Sci 76:4165-4178 (2019). PubMed: 31076805
Images
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Western blot - Anti-Cyclophilin A antibody (ab41684)All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab41684)
ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab41684) This image is courtesy of an abreview submitted by Alina Macovei.
Immunocytochemistry/ Immunofluorescence analysis of HepaRG cells labeling Cyclophilin A with ab41684 at 1μg/ml. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS. The cells were blocked with 1% milk for 20 minutes at room temperature. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/400 dilution.
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Western blot - Anti-Cyclophilin A antibody (ab41684)ab88202 is a GST-tagged recombinant protein with an expected molecular weight of 50kDa.
