Anti-Cyclophilin A antibody (ab42408)
Key features and details
- Rabbit polyclonal to Cyclophilin A
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclophilin A antibody
See all Cyclophilin A primary antibodies -
Description
Rabbit polyclonal to Cyclophilin A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Cow, Dog, Pig, Chimpanzee, Monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Cyclophilin A aa 1-100.
(Peptide available asab42407)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab42408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB ICC/IF Application notesICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at in the range of 1/100 - 1/500 depending on incubation time/ condtions of primary.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5478 Human
- Entrez Gene: 268373 Mouse
- Entrez Gene: 100360977 Rat
- Entrez Gene: 100362969 Rat
- Entrez Gene: 25518 Rat
- Omim: 123840 Human
- SwissProt: P62937 Human
- SwissProt: P17742 Mouse
see all -
Alternative names
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
see all
Images
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Western blot - Anti-Cyclophilin A antibody (ab42408)All lanes : Anti-Cyclophilin A antibody (ab42408) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa -
Western blot - Anti-Cyclophilin A antibody (ab42408) -
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab42408)ICC/IF image of ab42408 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab42408, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
References (6)
ab42408 has been referenced in 6 publications.
- Gläser A et al. Identification of Brain-Specific Treatment Effects in NPC1 Disease by Focusing on Cellular and Molecular Changes of Sphingosine-1-Phosphate Metabolism. Int J Mol Sci 21:N/A (2020). PubMed: 32599915
- White AG et al. Single blinded, randomized, placebo-controlled study on the effects of ciclosporin on cutaneous barrier function and immunological response in atopic beagles. Vet Immunol Immunopathol 197:93-101 (2018). Dog . PubMed: 29475513
- Lin AL et al. Rapamycin rescues vascular, metabolic and learning deficits in apolipoprotein E4 transgenic mice with pre-symptomatic Alzheimer's disease. J Cereb Blood Flow Metab 37:217-226 (2017). WB ; Mouse . PubMed: 26721390
- Halliday MR et al. Accelerated pericyte degeneration and blood-brain barrier breakdown in apolipoprotein E4 carriers with Alzheimer's disease. J Cereb Blood Flow Metab 36:216-27 (2016). PubMed: 25757756
- Heinzmann D et al. The Novel Extracellular Cyclophilin A (CyPA) - Inhibitor MM284 Reduces Myocardial Inflammation and Remodeling in a Mouse Model of Troponin I -Induced Myocarditis. PLoS One 10:e0124606 (2015). Histology ; Mouse . PubMed: 25894208
- Keyes LR et al. Cyclophilin A is required for efficient human cytomegalovirus DNA replication and reactivation. J Gen Virol 93:722-32 (2012). WB ; Human . PubMed: 22238232
Images
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Western blot - Anti-Cyclophilin A antibody (ab42408)All lanes : Anti-Cyclophilin A antibody (ab42408) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
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Western blot - Anti-Cyclophilin A antibody (ab42408)
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab42408)
ICC/IF image of ab42408 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab42408, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
