Anti-Polyethylene glycol antibody [RM105] (ab190652)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM105] to Polyethylene glycol
- Suitable for: ICC, WB, ELISA, IHC-P, Flow Cyt, IP
Overview
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Product name
Anti-Polyethylene glycol antibody [RM105]
See all Polyethylene glycol primary antibodies -
Description
Rabbit monoclonal [RM105] to Polyethylene glycol -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, ELISA, IHC-P, Flow Cyt, IPmore details -
Immunogen
Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to Keyhole Limpet Haemocyanin (KLH). (KLH-PEG with terminal methoxy group).
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Positive control
- Mouse liver and kidney tissue injected with PEG-BSA; PEGylated BSA.
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General notes
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody (RM105) reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody. This detergent can safely be used in ELISA assays.
Learn more about PEG RabMAb products and kit - PEG RabMAb Product Portal Page
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab190652 was purified from an animal origin–free and protein-free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM105 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Polyethylene glycol antibody [RM105] (ab190652) at 0.1 µg/ml
Lane 1 : BSA at 0.2 µg
Lane 2 : PEGylated BSA at 0.2 µg
Lane 3 : BSA at 0.1 µg
Lane 4 : PEGylated BSA at 0.1 µg
Lane 5 : BSA at 0.05 µg
Lane 6 : PEGylated BSA at 0.05 µg
Lane 7 : BSA at 0.02 µg
Lane 8 : PEGylated BSA at 0.02 µg
Lane 9 : BSA at 0.01 µg
Lane 10 : PEGylated BSA at 0.01 µg
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ELISA of three different PEGylated BSA using ab190652 at 0.3 µg/ml, following AP conjugated goat anti-rabbit IgG.
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Immunohistochemical analysis of Mouse liver and kidney tissue labeling PEG with ab190652 at 0.5 µg/ml. Mouse had been injected with PEG-BSA (left panels) or BSA (right panels) for 3 hours before sampling.
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