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Cell Biology Other Antibodies Drugs and Chemicals

Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

Price and availability

328 339 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [PEG-B-47b] to Polyethylene glycol
  • Suitable for: IHC-P, Sandwich ELISA, WB, ELISA
  • Conjugation: Biotin

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Overview

  • Product name

    Biotin Anti-Polyethylene glycol antibody [PEG-B-47b]
    See all Polyethylene glycol primary antibodies
  • Description

    Biotin Rabbit monoclonal [PEG-B-47b] to Polyethylene glycol
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-P, Sandwich ELISA, WB, ELISAmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to keyhole limpet haemocyanin. KLH-PEG with a terminal methoxy group.

  • Positive control

    • WB: BSA-PEG5K. IHC-P: Human cerebral cortex tissue
  • General notes

    The smallest PEG molecule which our Polyethylene glycol antibody (ab53449) can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing. 

    Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents when performing ELISA using anti-PEG products.

    Learn more about PEG RabMAb® primary antibodies and kit - PEG RabMAb Product Portal Page

    If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 2.41 x 10 -9 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    PEG-B-47b
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Drugs and Chemicals

Images

  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.

    Biotin conjugate (ab53449) available for this clone.

  • Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Sandwich ELISA antigen dose-response curve using purified ab53449.

    Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).

    Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).

    Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/50000 dilution (purified) + BSA-PEG5K at 0.02 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 2 kDa
    Observed band size: 280 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue showing negative staining with purified ab53449 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    ELISA assay using 5 µg/mL Anti-AFP RabMAb as the capture antibody and 0.5 µg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.

  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).

  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Lane 1 : Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/5000 dilution (unpurified)
    Lane 2 : Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/10000 dilution (unpurified)
    Lane 3 : Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/20000 dilution (unpurified)

    All lanes : BSA-PEG

    Predicted band size: 2 kDa

  • Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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