Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [PEG-B-47b] to Polyethylene glycol - BSA and Azide free
  • Suitable for: Sandwich ELISA, WB, IHC-P, ELISA

Overview

  • Product name

    Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free
    See all Polyethylene glycol primary antibodies

  • Description

    Rabbit monoclonal [PEG-B-47b] to Polyethylene glycol - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Sandwich ELISA, WB, IHC-P, ELISAmore details

  • Immunogen

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebral cortex tissue.

  • General notes

    The smallest PEG molecule which our antibody can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing. Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents when performing ELISA using anti-PEG products.

    Learn more about PEG RabMAb primary antibodies and kits - PEG RabMAb Product Portal Page. If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.

    ab271853 is the carrier-free version of ab53449. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 2.41 x 10 -9 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    PEG-B-47b

  • Isotype

    IgG

  • Research areas

Images

  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

    Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.

    Biotin conjugate (ab53449) available for this clone.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).
  • Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
    Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

    Sandwich ELISA antigen dose-response curve using purified ab53449.

    Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).

    Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).

    Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue showing negative staining with purified ab53449 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).
  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

    ELISA assay using 5 µg/mL Anti-AFP RabMAb as the capture antibody and 0.5 µg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).
  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

    Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).
  • Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
    Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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