Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [PEG-B-47] to Polyethylene glycol - BSA and Azide free
  • Suitable for: ELISA, WB, IHC-P
  • Reacts with: Species independent

Overview

  • Product name

    Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free
    See all Polyethylene glycol primary antibodies

  • Description

    Rabbit monoclonal [PEG-B-47] to Polyethylene glycol - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse kidney, spleen, muscle and liver tissue - (animals injected with a PEGylated protein).

  • General notes

    ab170969 is the carrier-free version of ab51257 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab170969 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 2.41 x 10 -9 M
    Learn more about KD

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    PEG-B-47

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

    This IHC data was generated using the same anti-PEG antibody clone, PEG-B-47, in a different buffer formulation (cat# ab51257).

    ab51257, unpurified,  staining mouse kidney at 1:10 dilution. Left panel: without animal injection with a PEGylated protein. Right panel: with animal injection with a PEGylated protein.

  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    ELISA assay using ab51257 to detect different forms of PEG. PEG40000-BSA is a 40 kDa PEG molecule attached to BSA. PEG5000-BSA is a 5 kDa linear PEG molecule attached to BSA.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

    ab51257, unpurified, staining mouse spleen at 1:10 dilution. Left panel: without animal injection with a PEGylated protein. Right panel: with animal injection with a PEGylated protein.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

    ab51257, unpurified, staining mouse muscle at 1:10 dilution. Left panel: without animal injection with a PEGylated protein. Right panel: with animal injection with a PEGylated protein.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

    ab51257, unpurified,  staining mouse liver at 1:10 dilution. Left panel: without animal injection with a PEGylated protein. Right panel: with animal injection with a PEGylated protein.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Functional Studies - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    Functional Studies - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

    This antibody affinity data was generated using the same anti-PEG antibody clone, PEG-B-47, in a different buffer formulation (ab51257).

    Method：Protein A sensor + Antigen (PEG 5K-BSA at 0.3µg/ml)+ antibody (ab51257 at  0.0312, 0.0625, 0.125, 0.25, 0.5, 1 µg/m).

    Results:

    Sample ID: PEG-5K-BSA

    KD (M): 2.41E-09

    kon(1/Ms): 2.67E+04

    kon Error: 6.81E+02

    kdis(1/s): 6.43E-05

    kdis Error: 2.92E-06

    Full X^2: 0.018444

    Full R^2: 0.982059

  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    Comparison of ab51257 and Vendor A mouse MAb in Direct ELISA assy.

    Goat anti-rabbit IgG-AP used for anti-PEG-47 detection; goat anti-mouse IgM-AP used for Vendor A MAb detection.

    Fig 1a. Direct ELISA using 1 ug/mL of PEG-GCSF.

    Fig 1b. Direct ELISA using 1 ug/mL of PEG-IFN.

    Comparison to other anti-PEG: In both direct and sandwich ELISA assays, ab51257 shows greater affinity and accuracy than other anti-PEG antibodies when determining the concentration of PEG or PEG-modified proteins. Results were similar whether detecting PEG itself or PEG-modified targets.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    Comparison of sandwich ELISA using RabMAb/RabMAb (ab51257/ab51257) and MAb/RabMAb (Vendor A/ab51257) for capture/detection.

    Ab51257/ab51257*: Plate coated with 5 ug/mL of #47; 5 ug/mL of #47 used for detection (*Anti-PEG 47 biotin labeled)

    Vendor A/ ab51257*: Plates coated with 100 ug/mL of Vendor A Mouse MAb; 5 ug/mL of #47 used for detection (*Anti-PEG 47 biotin labeled)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    Comparison of 10 ug/well of activated linear (PEG5K) and branched (PEG40K) PEG using 5 ug/ml of ab51257 in Direct ELISA assay.

    Accuracy: By detecting the methoxy group of the PEG molecule itself, ab51257 is useful in measuring the pharmacokinetics of PEG-modified molecules in vivo. Data indicate that ab51257 detects various length Y-chain PEG molecules as well as single chain PEG molecules with equal affinity. Ab51257 does not cross react with non-specific targets in blood or serum.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

    This data was developed using ab51257, the same antibody clone in a different buffer formulation.

    ELISA Graph generated using purified ab51257 at 1μg/mL.

    Antigen concentration range:

    PEG5000 and PEG20000: 0.0078 - 0.5 μg/mL
    ADI-PEG: 0.0156 - 1 μg/mL
    PEG-BSA: 0.0039 - 0.25μg/mL
    PEG-IFN-α-2b0.0156 - 1 μg/mL

    Secondary antibody was an Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG(H+L) at 1/2500.

  • Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)
    Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

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