Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

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Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21993-154] to Polyethylene glycol
Suitable for: WB, ELISA
Reacts with: Species independent

Product name

Anti-Polyethylene glycol antibody [EPR21993-154]
See all Polyethylene glycol primary antibodies
Description

Rabbit monoclonal [EPR21993-154] to Polyethylene glycol
Host species

Rabbit
Tested Applications & Species

Application Species
See all applications and species data
Immunogen

Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: BSA-methoxy PEG 5K lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21993-154
Isotype

IgG
Research areas

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/1000 dilution + BSA-methoxy PEG 5K, 10 ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 250 kDa
why is the actual band size different from the predicted?

Exposure time: 92 seconds.

Blocking/Diluting buffer: 5% NFDM/TBST.
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Direct ELISA antigen dose-response curve using AffniPure Goat Anti-Mouse IgG (H&L) at 1 µg/ml concentration. The detector antibody was ab203857 anti-Polyethylene glycol at 1 µg/ml. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range: 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

Washing Buffer: 1 X TBST.

Substrate: p-nitrophenyl phosphate (PNPP).

Coloration time: 10 min.
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

Antigen concentration range: 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

Washing Buffer: 1 X TBST.

Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).

Coloration time: 2 min.
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

Antigen concentration range: 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

Washing Buffer: 1 X TBST.

Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).

Coloration time: 2 min.
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range: 0-0.05µg/ml.

Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

Washing Buffer: 1 X TBST.

Substrate: p-nitrophenyl phosphate (PNPP).

Coloration time: 15 min.
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range: 0-0.05µg/ml.

Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

Washing Buffer: 1 X TBST.

Substrate: p-nitrophenyl phosphate (PNPP).

Coloration time: 15 min.
Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Method: APS sensor+ Antigen (methoxy PEG800 at 0.625 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 2, 1, 0.5, 0.25, 0.125µg/ml).

Results:

KD (M) = 5.81E-11

kon(1/Ms) = 2.70E+05

kon Error = 6.28E+02

kdis(1/s) = 1.57E-05

kdis Error = 4.49E-07

Full X²= 10.143

Full R²= 0.9993

Testing Biosensor: Aminopropylsilane (APS) Biosensors

Testing machine: Fortebio RED96e
Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Method: APS sensor+ Antigen (methoxy PEG800 at 25 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 0.5, 0.25, 0.125, 0.0625, 0.03125µg/ml).

Results:

KD (M) = 1.29E-10

kon(1/Ms) = 2.23E+06

kon Error = 1.45E+04

kdis(1/s) = 2.88E-04

kdis Error = 1.74E-06

Full X² = 3.475

Full R² = 0.9865

Testing Biosensor: Aminopropylsilane (APS) Biosensors

Testing machine: Fortebio RED96e
Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

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