Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21993-154] to Polyethylene glycol
  • Suitable for: WB, ELISA
  • Reacts with: Species independent

Overview

  • Product name

    Anti-Polyethylene glycol antibody [EPR21993-154]
    See all Polyethylene glycol primary antibodies

  • Description

    Rabbit monoclonal [EPR21993-154] to Polyethylene glycol

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    See all applications and species data

  • Immunogen

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BSA-methoxy PEG 5K lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21993-154

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/1000 dilution + BSA-methoxy PEG 5K, 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 250 kDa
    why is the actual band size different from the predicted?



    Exposure time: 92 seconds.

    Blocking/Diluting buffer: 5% NFDM/TBST.

  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using AffniPure Goat Anti-Mouse IgG (H&L) at 1 µg/ml concentration. The detector antibody was ab203857 anti-Polyethylene glycol at 1 µg/ml. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Antigen concentration range: 0-1000 ng/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: p-nitrophenyl phosphate (PNPP).

    Coloration time: 10 min.

  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

    Antigen concentration range: 0-1000 ng/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).

    Coloration time: 2 min.

  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

    Antigen concentration range: 0-1000 ng/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).

    Coloration time: 2 min.

  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Antigen concentration range: 0-0.05µg/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: p-nitrophenyl phosphate (PNPP).

    Coloration time: 15 min.

  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Antigen concentration range: 0-0.05µg/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: p-nitrophenyl phosphate (PNPP).

    Coloration time: 15 min.

  • Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Method: APS sensor+ Antigen (methoxy PEG800 at 0.625 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 2, 1, 0.5, 0.25, 0.125µg/ml).

    Results:

    KD (M) = 5.81E-11

    kon(1/Ms) = 2.70E+05

    kon Error = 6.28E+02

    kdis(1/s) = 1.57E-05

    kdis Error = 4.49E-07

    Full X2= 10.143

    Full R2= 0.9993

    Testing Biosensor: Aminopropylsilane (APS) Biosensors

    Testing machine: Fortebio RED96e

  • Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Method: APS sensor+ Antigen (methoxy PEG800 at 25 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 0.5, 0.25, 0.125, 0.0625, 0.03125µg/ml).

    Results:

    KD (M) = 1.29E-10

    kon(1/Ms) = 2.23E+06

    kon Error = 1.45E+04

    kdis(1/s) = 2.88E-04

    kdis Error = 1.74E-06

    Full X2 = 3.475

    Full R2 = 0.9865

    Testing Biosensor: Aminopropylsilane (APS) Biosensors

    Testing machine: Fortebio RED96e

  • Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

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