Astana Biomed Group, an authorized Abcam distributor in Central Asia

Prostaglandin E1 ELISA Kit (ab133024)

Key features and details

  • Sensitivity: 5.58 pg/ml
  • Range: 4.88 pg/ml - 5000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human

Overview

  • Product name

    Prostaglandin E1 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low 24 53pg/ml 4.6%
    Medium 24 246pg/ml 9.5%
    High 24 1103pg/ml 13.7%
    Inter-assay
    Sample n Mean SD CV%
    Low 8 49pg/ml 9.3%
    Medium 8 214pg/ml 11%
    High 8 737pg/ml 6.2%

  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Plasma, Tissue Culture Media

  • Assay type

    Competitive

  • Sensitivity

    5.58 pg/ml

  • Range

    4.88 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 107.2 % - %
    Urine 109.9 % - %
    Serum 87 % - %
    Plasma 107.7 % - %
    Tissue Culture Media 90% - 110%

  • Assay time

    3h 0m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species

  • Product overview

    Abcam’s Prostaglandin E1in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E1 in, plasma, serum, urine, tissue culture media and other biological fluids.

    A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGE1 antigen and a polyclonal sheep antibody specific to PGE1. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the Alkaline Phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGE1 captured in the plate.

  • Notes

    Prostaglandin E1 (PGE1) is synthesized from dihomo-γ-linolenic acid (DGLA). PGE1 has been shown to have a number of biological actions, including vasodilation, proliferation of vascular smooth muscle cells, platelet aggregation and has been shown to have insulin-like actions. Its effects are induced by receptor mediated elevation of cAMP. PGE1 is the major prostaglandin in semen.

    Cross Reactivity

    CompoundCross Reactivity
     PGE1100%
     PGE26.50%
     PGE32.22%
     13,14-dihydro-PGE11.50%
     PGE01.45%
     15-keto-PGE11.15%
     13,14-dihydro-15-keto-PGE10.19%
     PGF1α0.14%
     PGF2α0.04%
     6-keto-PGF1α
     PGA2
     PGD2
     PGB1
     13,14-dihydro-15-keto-PGF2α
     6,15-keto-13,14-dihydro-PGF1α
     Thromboxane B2
     Misoprostol
     2-Arachidonoylglycerol
     Anandamide

  • Platform

    Microplate

Properties

Images

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab133024

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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