Prostaglandin E1 ELISA Kit (ab133024)
Key features and details
- Sensitivity: 5.58 pg/ml
- Range: 4.88 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Prostaglandin E1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low 24 53pg/ml 4.6% Medium 24 246pg/ml 9.5% High 24 1103pg/ml 13.7% Inter-assay Sample n Mean SD CV% Low 8 49pg/ml 9.3% Medium 8 214pg/ml 11% High 8 737pg/ml 6.2% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Plasma, Tissue Culture Media -
Assay type
Competitive -
Sensitivity
5.58 pg/ml -
Range
4.88 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 107.2 % - % Urine 109.9 % - % Serum 87 % - % Plasma 107.7 % - % Tissue Culture Media 90% - 110% -
Assay time
3h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species
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Product overview
Abcam’s Prostaglandin E1in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E1 in, plasma, serum, urine, tissue culture media and other biological fluids.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGE1 antigen and a polyclonal sheep antibody specific to PGE1. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the Alkaline Phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGE1 captured in the plate.
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Notes
Prostaglandin E1 (PGE1) is synthesized from dihomo-γ-linolenic acid (DGLA). PGE1 has been shown to have a number of biological actions, including vasodilation, proliferation of vascular smooth muscle cells, platelet aggregation and has been shown to have insulin-like actions. Its effects are induced by receptor mediated elevation of cAMP. PGE1 is the major prostaglandin in semen.
Cross Reactivity
Compound Cross Reactivity PGE1 100% PGE2 6.50% PGE3 2.22% 13,14-dihydro-PGE1 1.50% PGE0 1.45% 15-keto-PGE1 1.15% 13,14-dihydro-15-keto-PGE1 0.19% PGF1α 0.14% PGF2α 0.04% 6-keto-PGF1α PGA2 PGD2 PGB1 13,14-dihydro-15-keto-PGF2α 6,15-keto-13,14-dihydro-PGF1α Thromboxane B2 Misoprostol 2-Arachidonoylglycerol Anandamide -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit PGE1 Alkaline Phosphatase Conjugate 1 x 5ml PGE1 Standard 1 x 500µl PGE1 Antibody 1 x 5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
Images
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Typical Standard Curve
Representative Standard Curve using ab133024
