Pyrophosphate Assay Kit II (Fluorometric) (ab179836)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate
- Sensitivity: 1 µM
Overview
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Product name
Pyrophosphate Assay Kit II (Fluorometric)
See all Pyrophosphate kits -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
>= 1 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Pyrophosphate (PPi) are produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.
Pyrophosphate Assay Kit II (Fluorometric) (ab179836) provides the most robust spectrophotometric method for the measurement of pyrophosphate. It uses our proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. Our assay is much easier and more robust than enzyme-coupling pyrophosphate methods, which require at least two enzymes for their pyrophosphate detections. Due to its direct measurement of pyrophosphate, this kit is ideal for screening inhibition or activities of enzymes that consume or generate pyrophosphate with enhanced selectivity to pyrophosphate. The assay is an optimized mix-and-read assay and can be performed in a convenient 96-well or 384-well microtiter-plate format. The kit provides all the essential components for assaying pyrophosphate.
Assay time: 10-30 minutes
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Notes
This product measures PPi formation at Ex/Em = 370/470 nm.
If your instrument has filters for Ex/Em = 316/456 nm, we recommend using Pyrophosphate Assay Kit (Fluorometric) (ab112155).
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 25ml DMSO 1 x 100µl PPi Sensor 1 vial Pyrophosphate Standard (50mM) 1 x 1ml -
Research areas
Images
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Functional assay - 179836 Pyrophosphate Assay Kit II (Fluorometric)
Pyrophosphate measured in mouse tissue lysates showing quantities (nanomoles) per microgram of protein tested. The lysates were tested in the range of 2-50 microgram of proteins per mL, data shown from duplicates, +/- SD.
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Functional assay - 179836 Pyrophosphate Assay Kit II (Fluorometric)Pyrophosphate measured in cell lysates showing quantity (micromoles) per 1 million cells tested.
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Functional assay - 179836 Pyrophosphate Assay Kit II (Fluorometric)Pyrophosphate measured in biological fluids showing concentration (millimolar). The samples were tested in the dilution range of 1:200-1:5000.
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Pyrophosphate standard curvePyrophosphate, ATP and phosphate dose responses were measured with the Abcam Pyrophosphate Assay Kit II (Fluorometric) (ab179836) in a solid black 96-well plate using a fluorescence microplate reader. As low as 1 µM (100 picmoles/well) pyrophosphate can be detected with 10 minutes incubation.
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Excitation and Emission Spectra for Pyrophosphate Assay Kit II (Fluorometric) (ab179836)
