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Cell Biology Other Antibodies Drugs and Chemicals

Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)

Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [EPR21993-154] to Polyethylene glycol - Chimeric
  • Suitable for: WB, ELISA
  • Reacts with: Species independent

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Overview

  • Product name

    Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric
    See all Polyethylene glycol primary antibodies
  • Description

    Mouse monoclonal [EPR21993-154] to Polyethylene glycol - Chimeric
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BSA-methoxy PEG 5K.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.04% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21993-154
  • Isotype

    IgG2b
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Drugs and Chemicals

Images

  • Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)
    Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)
    Lane 1 : Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332) at 1/5000 dilution
    Lane 2 : Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/5000 dilution

    All lanes : BSA-methoxy PEG 5K, 10 ng

    Secondary
    Lane 1 : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Observed band size: 250 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)
    ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)

    ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab275332, 50 ng/ml).

    Antigen used at 0-500ng: NH2-PEG 5K-OCH3, NH2-PEG20K-NH2, PEG1000, PEG1000-BSA, mPEG800, mPEG800-BSA.

    Secondary antibody used at 1/1000: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H&L).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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