Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EPR21993-154] to Polyethylene glycol - Chimeric
- Suitable for: WB, ELISA
- Reacts with: Species independent
Overview
-
Product name
Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric
See all Polyethylene glycol primary antibodies -
Description
Mouse monoclonal [EPR21993-154] to Polyethylene glycol - Chimeric -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: BSA-methoxy PEG 5K.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21993-154 -
Isotype
IgG2b -
Research areas
Images
-
Lane 1 : Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332) at 1/5000 dilution
Lane 2 : Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/5000 dilution
All lanes : BSA-methoxy PEG 5K, 10 ng
Secondary
Lane 1 : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab275332, 50 ng/ml).
Antigen used at 0-500ng: NH2-PEG 5K-OCH3, NH2-PEG20K-NH2, PEG1000, PEG1000-BSA, mPEG800, mPEG800-BSA.
Secondary antibody used at 1/1000: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H&L).