Leukotriene B4 ELISA Kit (ab133040)
Key features and details
- Sensitivity: 5.63 pg/ml
- Range: 11.7 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human, Pig
Overview
-
Product name
Leukotriene B4 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Medium 607pg/ml 6.8% High 1078pg/ml 5.9% Low 305pg/ml 6% Inter-assay Sample n Mean SD CV% Low 99pg/ml 15.7% Medium 308pg/ml 16.5% High 507pg/ml 5.5% -
Sample type
Cell culture supernatant, Saliva, Urine, Plasma -
Assay type
Competitive -
Sensitivity
5.63 pg/ml -
Range
11.7 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 114.1 % - % Urine 96.9 % - % Plasma 109.6 % - % Tissue Culture Media 97.3 % - % -
Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human, Pig -
Product overview
Abcam’s Leukotriene B4 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Leukotriene B4 (LTB4) in tissue culture media, plasma, urine and saliva.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-LTB4 antigen and a polyclonal rabbit antibody specific to LTB4. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of LTB4 captured in the plate.
-
Notes
Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.
Cross Reactivity
Compound Cross Reactivity % LTB4 100 6-trans-12-epi-LTB4 5.50 12-epi-LTB4 4.90 PGE2 0.94 PGF2α 20-OH-LTB4 20-COOH-LTB4 LTC4 LTD4 LTE4 5(S)-HETE 12(S)-HETE 15(S)-HETE -
Platform
Microplate
Properties
-
Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Leukotriene B4 Alkaline Phosphatase Conjugate 1 x 5ml Leukotriene B4 Antibody 1 x 5ml Leukotriene B4 Standard 1 x 500µl Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
-
Alternative names
- Leukotriene B4
Images
-
Typical Standard Curve
Representative Standard Curve using ab133040