Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric – BSA and Azide free (ab275333)
![Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric – BSA and Azide free (ab275333)](https://www.abcam.com/ps/products/275/ab275333/Images/ab275333-403586-AntiPolyethylene-glycol-antibody-EPR21993154-western-blot-bsamethoxy-peg.jpg "Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric – BSA and Azide free (ab275333)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EPR21993-154] to Polyethylene glycol - Chimeric – BSA and Azide free
- Suitable for: WB, ELISA
- Reacts with: Species independent
Overview
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Product name
Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric – BSA and Azide free
See all Polyethylene glycol primary antibodies -
Description
Mouse monoclonal [EPR21993-154] to Polyethylene glycol - Chimeric – BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: BSA-methoxy PEG 5K.
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General notes
ab275333 is the carrier-free version of ab275332. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275333 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21993-154 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric – BSA and Azide free (ab275333)Lane 1 : Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332) at 1/5000 dilution
Lane 2 : Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/5000 dilution
All lanes : BSA-methoxy PEG 5K, 10 ng
Secondary
Lane 1 : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab275332, the same antibody clone in a different buffer formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric – BSA and Azide free (ab275333)](https://www.abcam.com/ps/products/275/ab275333/Images/ab275333-403585-AntiPolyethylene-glycol-antibody-EPR21993154-elisa.jpg)
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric – BSA and Azide free (ab275333)This data was developed using ab275332, the same antibody clone in a different buffer formulation.
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab275332, 50 ng/ml).
Antigen used at 0-500ng: NH2-PEG 5K-OCH3, NH2-PEG20K-NH2, PEG1000, PEG1000-BSA, mPEG800, mPEG800-BSA.
Secondary antibody used at 1/1000: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H&L).