Native human MMP9/ TIMP1/Lipocalin protein (Complex) (ab134445)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, Inhibition Assay, SDS-PAGE
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Product name
Native human MMP9/ TIMP1/Lipocalin protein (Complex) -
Biological activity
The specific activity of ab134445 after trypsin activation is >600 mU/mg. 1 U is the activity that hydrolyzes 1 µmol peptide (7-methoxycoumarin-4-yl) acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-initrophenyl]-L- 2, 3-diamino-propionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-GlyLeu-Dpa-Ala-Arg) within 1 minute. -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Predicted molecular weight
130 kDa
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 0.05% Sodium azide
Constituents: 0.05% Brij, 0.05% Calcium chloride, 0.0001% Zinc chloride, 0.79% Tris HCl, 1.17% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Native human MMP9/ TIMP1/Lipocalin protein (Complex) (ab134445)SDS-PAGE (reducing) analysis of ab134445.
ab134445 consists of 668 amino acids with a calculated MWt of 76 kDa. Due to N- and O-linked glycosylation the MWt in SDS-PAGE is about 92 kDa. MMP9/TIMP1/Lipocalin from neutrophil granulocytes displays three bands on SDS-PAGE at 92, 29 and 25 kDa. The 92 kDa form represents the monomer, the 29 kDa protein band represents TIMP 1 and the 25 kDa Lipocalin.
Lane 1: MMP9
Lane 2: MMP9/TIMP1/Lipocalin
Lane 3: MMP9/TIMP1
