Anti-Cyclophilin A antibody (ab58144)
Key features and details
- Mouse monoclonal to Cyclophilin A
- Suitable for: WB, ICC/IF, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Cyclophilin A antibody
See all Cyclophilin A primary antibodies -
Description
Mouse monoclonal to Cyclophilin A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Cyclophilin A aa 1-165.
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General notes
This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Cyclophilin A antibody (ab58144)
Cyclophilin A antibody (ab58144) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab58144)ICC/IF image of ab58144 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58144, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Immunoprecipitation - Anti-Cyclophilin A antibody (ab58144)Cyclophilin A was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Mouse monoclonal to Cyclophilin A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58144.
Secondary: Protein G-HRP at 1/500 dilution.
Band: 18kDa: Cyclophilin A.This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-Cyclophilin A antibody (ab58144)Overlay histogram showing HeLa cells stained with ab58144 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58144, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
