Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
![Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)](https://www.abcam.com/ps/products/250/ab250754/Images/ab250754-410430-anti-ppic-antibody-epr15355-bsa-and-azide-free-western-blot.jpg "Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15355] to PPIC - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-PPIC antibody [EPR15355] - BSA and Azide free
See all PPIC primary antibodies -
Description
Rabbit monoclonal [EPR15355] to PPIC - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung carcinoma tissue; WB: HeLa, U87-MG and Human fetal kidney lysates; U87-MG cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Ab250754 is the carrier-free version of ab184552. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250754 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15355 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)All lanes : Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/10000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDaThis data was developed using ab184552, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)](https://www.abcam.com/ps/products/250/ab250754/Images/ab250754-4-anti-ppic-antibody-epr15355-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)This data was developed using ab184552, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)](https://www.abcam.com/ps/products/250/ab250754/Images/ab250754-406118-anti-ppic-antibody-epr15355-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/50000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDaThis data was developed using ab184552, the same antibody clone in a different buffer formulation.
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![Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)](https://www.abcam.com/ps/products/250/ab250754/Images/ab250754-6-benefits-of-recombinant-antibodies.png)
Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
