Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15355] to PPIC - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PPIC antibody [EPR15355] - BSA and Azide free
    See all PPIC primary antibodies

  • Description

    Rabbit monoclonal [EPR15355] to PPIC - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung carcinoma tissue; WB: HeLa, U87-MG and Human fetal kidney lysates; U87-MG cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Ab250754 is the carrier-free version of ab184552. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250754 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
    Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
    All lanes : Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/10000 dilution

    Lane 1 : U-87 MG cell lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa



    This data was developed using ab184552, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
    This data was developed using ab184552, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
    Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
    Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/50000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa



    This data was developed using ab184552, the same antibody clone in a different buffer formulation.

  • Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
    Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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