Anti-CD276 antibody [BLR026F] - BSA free (ab243883)
![Anti-CD276 antibody [BLR026F] - BSA free (ab243883)](https://www.abcam.com/ps/products/243/ab243883/Images/ab243883-334324-anti-cd276-antibody-blr026f-immunoprecipitation.jpg "Anti-CD276 antibody [BLR026F] - BSA free (ab243883)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR026F] to CD276 - BSA free
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-CD276 antibody [BLR026F] - BSA free
See all CD276 primary antibodies -
Description
Rabbit monoclonal [BLR026F] to CD276 - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human CD276 (extracellular).
Database link: Q5ZPR3 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR026F -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CD276 antibody [BLR026F] - BSA free (ab243883)CD276 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243883 at 3 µl per reaction. Western blot was performed on the immunoprecipitate using ab243883 at 1/1000 dilution.
Lane 1: ab243883 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
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![Western blot - Anti-CD276 antibody [BLR026F] - BSA free (ab243883)](https://www.abcam.com/ps/products/243/ab243883/Images/ab243883-334323-anti-cd276-antibody-blr026f-western-blot.jpg)
Western blot - Anti-CD276 antibody [BLR026F] - BSA free (ab243883)Western blot analysis using ab243883 at 1/1000 dilution.
Lane 1: MCF7 whole cell lysate.
Lane 2: A172 whole cell lysate.
Lane3: U-251 MG whole cell lysate.
Lane 4: OVCAR-3 whole cell lysate.
Lane 5: HeLa whole cell lysate.
Lane 6: HEK-293T whole cell lysate.
Lane 7: HCT 116 whole cell lysate.
Lane 8: RPMI 8226 whole cell lysate.
Lane 9: MJ whole cell lysate.
Lane 10: Ramos whole cell lysate.
Lane 11: Jurkat whole cell lysate.
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence.
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![Immunocytochemistry - Anti-CD276 antibody [BLR026F] - BSA free (ab243883)](https://www.abcam.com/ps/products/243/ab243883/Images/ab243883-334322-anti-cd276-antibody-blr026f-immunocytochemistry.jpg)
Immunocytochemistry - Anti-CD276 antibody [BLR026F] - BSA free (ab243883)Formalin-fixed, paraffin-embedded A172 cells labeling CD276 using ab243883 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [BLR026F] - BSA free (ab243883)](https://www.abcam.com/ps/products/243/ab243883/Images/ab243883-334321-anti-cd276-antibody-blr026f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [BLR026F] - BSA free (ab243883)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CD276 using ab243883 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining. -
![Anti-CD276 antibody [BLR026F] - BSA free (ab243883)](https://www.abcam.com/ps/products/243/ab243883/Images/ab243883-5-benefits-of-recombinant-antibodies.png)
Anti-CD276 antibody [BLR026F] - BSA free (ab243883)
