ab227679 staining CD276 in wild-type HEK293 cells (top panel) and CD276 knockout HEK293 cells (ab266658) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab227679 at 1/100 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor^® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor^® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Anti-CD276 antibody [SP265] - C-terminal (ab227679) at 1/100 dilution + Hs 700T (human pancreatic adenocarcinoma cell line) cell lysate

Developed using the ECL technique.

Predicted band size: 57 kDa

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling CD276  with purified ab227679 at 1/25 (9.24 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

Flow cytometric analysis of PC-3 (human prostate adenocarcinoma cell line) cell line labeling CC276 with ab227679 at 1/400 (green) compared with a rabbit isotype control (blue).

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human liver hepatoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human skin melanoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.