Anti-HLA-DQA1 antibody [EPR7300] (ab128959)
![Anti-HLA-DQA1 antibody [EPR7300] (ab128959)](https://www.abcam.com/ps/products/128/ab128959/Images/ab128959-142862-anti-hla-dqa1-antibody-epr7300-immunohistochemistry.jpg "Anti-HLA-DQA1 antibody [EPR7300] (ab128959)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7300] to HLA-DQA1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-HLA-DQA1 antibody [EPR7300]
See all HLA-DQA1 primary antibodies -
Description
Rabbit monoclonal [EPR7300] to HLA-DQA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human HLA-DQA1 aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- Human lymph node, spleen, tonsil, Daudi, and Raji cell lysates; Human colon and tonsil tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7300 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (ab128959)
ab128959, at 1/100 dilution, staining HLA-DQA1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-HLA-DQA1 antibody [EPR7300] (ab128959)](https://www.abcam.com/ps/products/128/ab128959/Images/ab128959-142864-anti-hla-dqa1-antibody-epr7300-western-blot.jpg)
Western blot - Anti-HLA-DQA1 antibody [EPR7300] (ab128959)All lanes : Anti-HLA-DQA1 antibody [EPR7300] (ab128959) at 1/10000 dilution
Lane 1 : Human lymph node lysate
Lane 2 : Daudi cell lysate
Lane 3 : Human spleen lysate
Lane 4 : Raji cell lysate
Lane 5 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 33-35 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (ab128959)](https://www.abcam.com/ps/products/128/ab128959/Images/ab128959-142863-anti-hla-dqa1-antibody-epr7300-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (ab128959)ab128959, at 1/100 dilution, staining HLA-DQA1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-HLA-DQA1 antibody [EPR7300] (ab128959)](https://www.abcam.com/ps/products/128/ab128959/Images/ab128959-142861-anti-hla-dqa1-antibody-epr7300.jpg)
OI-RD Scanning - Anti-HLA-DQA1 antibody [EPR7300] (ab128959) -
![Anti-HLA-DQA1 antibody [EPR7300] (ab128959)](https://www.abcam.com/ps/products/128/ab128959/Images/ab128959-5-benefits-of-recombinant-antibodies.png)
Anti-HLA-DQA1 antibody [EPR7300] (ab128959)
