Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human DKK1 protein (ab82115)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant human DKK1 protein
    See all DKK1 proteins and peptides

  • Biological activity

    Determined by its ability to inhibit mWnt3a-induced TCF/LEF-luciferase activity in reporter HEK293 cells.

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 97% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).

  • Endotoxin level

  • Expression system

    HEK 293 cells

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH

    • Specifications

    Our Abpromise guarantee covers the use of ab82115 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      For lot specific reconstitution information please contact our Scientific Support Team.

    General Info

    • Alternative names

      • Dickkopf 1
      • Dickkopf 1 homolog
      • Dickkopf 1 like
      • Dickkopf homolog 1
      • Dickkopf like protein 1
      • Dickkopf related protein 1
      • Dickkopf WNT signaling pathway inhibitor 1
      • Dickkopf-1
      • Dickkopf-related protein 1
      • DKK 1
      • Dkk-1
      • Dkk1
      • DKK1_HUMAN
      • hDkk 1
      • hDkk-1
      • SK
      see all

    • Function

      Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

    • Tissue specificity

      Placenta.

    • Sequence similarities

      Belongs to the dickkopf family.

    • Domain

      The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

    • Cellular localization

      Secreted.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab82115? Please let us know so that we can cite the reference in this datasheet.

ab82115 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

Images

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