Anti-FKBP12 antibody (ab24373)
Key features and details
- Rabbit polyclonal to FKBP12
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-FKBP12 antibody
See all FKBP12 primary antibodies -
Description
Rabbit polyclonal to FKBP12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human FKBP12 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab25840)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications CowPigApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).Target
-
Function
May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
Contains 1 PPIase FKBP-type domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 2280 Human
- Entrez Gene: 14225 Mouse
- Entrez Gene: 25639 Rat
- Omim: 186945 Human
- SwissProt: P18203 Cow
- SwissProt: P62942 Human
- SwissProt: P26883 Mouse
- SwissProt: Q62658 Rat
see all -
Alternative names
- 12 kDa FK506-binding protein antibody
- 12 kDa FKBP antibody
- Calstabin 1 antibody
see all
Images
-
Western blot - Anti-FKBP12 antibody (ab24373)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP1A knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole tissue lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab24373 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab24373 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP1A knockout cells. Wild-type and FKBP1A knockout samples were subjected to SDS-PAGE. ab24373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-FKBP12 antibody (ab24373)All lanes : Anti-FKBP12 antibody (ab24373) at 1 µg/ml
Lane 1 : Human Brain Tissue Lysate
Lane 2 : Mouse Brain Tissue Lysate
Lane 3 : Rat Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa -
Western blot - Anti-FKBP12 antibody (ab24373)
References (3)
ab24373 has been referenced in 3 publications.
- Tan Y & Kagan JC Innate Immune Signaling Organelles Display Natural and Programmable Signaling Flexibility. Cell 177:384-398.e11 (2019). PubMed: 30853218
- Nabet B et al. The dTAG system for immediate and target-specific protein degradation. Nat Chem Biol 14:431-441 (2018). PubMed: 29581585
- Hofhuis J et al. Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy. J Cell Sci 130:841-852 (2017). PubMed: 28104817
Images
-
Western blot - Anti-FKBP12 antibody (ab24373)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP1A knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole tissue lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab24373 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab24373 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP1A knockout cells. Wild-type and FKBP1A knockout samples were subjected to SDS-PAGE. ab24373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-FKBP12 antibody (ab24373)All lanes : Anti-FKBP12 antibody (ab24373) at 1 µg/ml
Lane 1 : Human Brain Tissue Lysate
Lane 2 : Mouse Brain Tissue Lysate
Lane 3 : Rat Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
-
Western blot - Anti-FKBP12 antibody (ab24373)
