Rat Dkk-1 ELISA Kit (ab136938)
Key features and details
- Sensitivity: 26.1 pg/ml
- Range: 39.1 pg/ml - 1250 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Dkk-1 ELISA Kit
See all DKK1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Buffer 20 488pg/ml 7.9% Buffer 20 286pg/ml 5% Buffer 20 163pg/ml 11.2% Inter-assay Sample n Mean SD CV% Buffer 485pg/ml 5.1% Buffer 263pg/ml 10.6% Buffer 131pg/ml 14% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
26.1 pg/ml -
Range
39.1 pg/ml - 1250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 98 100pg/ml - 800pg/ml EDTA Plasma 96 100pg/ml - 800pg/ml -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s Dkk-1 in vitro Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Dkk-1 in plasma, serum and culture fluids.
A monoclonal antibody specific for Dkk-1 has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A Dkk-1 polyclonal antibody is then added, incubated and washed. An HRP conjugated antibody is then added incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
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Notes
Compound Cross reactivity rat Dkk-1 100% mouse Dkk-2
≤0.03%
mouse Dkk-4
≤0.03%
mouse Sgy-1
≤0.03%
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Anti-mouse monoclonal Microplate (12 x 8 wells) 1 unit Assay Buffer 36 1 x 100ml Dkk-1 Antibody 1 x 10ml Dkk-1 Horseradish Peroxidase Conjugate 1 x 10ml Dkk-1 Rat Standard 1 x 2 vials Plate Sealer 2 units Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Relevance
DKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. -
Alternative names
- Dickkopf 1
- Dickkopf homolog 1
- Dickkopf related protein 1
see all -
Database links
Images
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Typical Standard Curve
Representative standard curve using ab136938.
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Typical Parallelism GraphA parallelism experiment was carried out to determine if the recombinant Dkk-1 standard accurately measures Dkk-1 concentrations in biological matrices. To assess parallelism, values for serum were obtained from a standard curve using four parameter logistic curve fitting. The observed concentration was plotted against the dilution factor. Parallelism indicates antibody-binding characteristics of the native and standard proteins are similar, allowing accurate determination of analyte.