Anti-CD276 antibody (ab232791)
Key features and details
- Rabbit polyclonal to CD276
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD276 antibody
See all CD276 primary antibodies -
Description
Rabbit polyclonal to CD276 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human CD276 aa 1-250. N-terminal tag. Expressed in E. coli.
Database link: Q5ZPR3 -
Positive control
- WB: Recombinant human CD276 protein. HEK-293T, MCF7 and PC-3 cell lysate. IHC-P: Human liver cancer, prostate and kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD276 antibody (ab232791)All lanes : Anti-CD276 antibody (ab232791) at 3 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Secondary
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody (ab232791)Formalin-fixed, paraffin-embedded human prostate tissue stained for CD276 with ab232791 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody (ab232791)Formalin-fixed, paraffin-embedded human kidney tissue stained for CD276 with ab232791 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody (ab232791)Formalin-fixed, paraffin-embedded human liver tissue stained for CD276 with ab232791 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-CD276 antibody (ab232791)Anti-CD276 antibody (ab232791) at 3 µg/ml + Recombinant human CD276 protein.
