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Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14845(B)] to Cyclophilin 40
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Cyclophilin 40 antibody [EPR14845(B)]
    See all Cyclophilin 40 primary antibodies

  • Description

    Rabbit monoclonal [EPR14845(B)] to Cyclophilin 40

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, JAR, HepG2 and K-562 cell lysate; Human fetal brain lysate; Recombinant Human Cyclophilin 40 protein (BSA and azide free) (ab173064). IHC-P: Human transitional cell carcinoma of bladder. ICC/IF: K562 cell lyaste.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14845(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)
    Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)
    All lanes : Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PPID knockout HeLa cell lysate
    Lane 3 : K-562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 41 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab181983 observed at 47 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab181983 Anti-Cyclophilin 40 antibody [EPR14845(B)] was shown to specifically react with Cyclophilin 40 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265788 (knockout cell lysate ab257600) was used.  Wild-type and Cyclophilin 40 knockout samples were subjected to SDS-PAGE.  ab181983 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)
    Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)
    All lanes : Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983) at 1/20000 dilution

    Lane 1 : Human fetal brain
    Lane 2 : JAR
    Lane 3 : K562
    Lane 4 : HepG2

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)

    Immunofluorescence staining of K562 cells labeling Cyclophilin 40 with ab181983 at 1/200 dilution followed by labeling with Goat
    anti rabbit IgG(Alexa Fluor® 555) antibody (1/500 dilution) and counter-staining with Dapi (blue)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)

    Imunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder labeling Cyclophilin 40 with ab181983 at 1/50 dilution followed by labeing with secondary Ready to use HRP Polymer for Rabbit IgG and hematoxylin staining.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)
    Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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