Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-336-1B2] to CD3 zeta
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-CD3 zeta antibody [BL-336-1B2]
See all CD3 zeta primary antibodies
Description

Rabbit monoclonal [BL-336-1B2] to CD3 zeta
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD3 zeta aa 150-164 (C terminal). The exact sequence is proprietary. NP_932170.1 and Gene ID 919.
Database link: P20963
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-336-1B2
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)

CD3 zeta was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243874 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243874 at 1/1000 dilution.
Lane 1: ab243874 IP in Jurkat whole cell lysate.

Lane 2: Contol IgG in Jurkat whole cell lysate.

Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)

Western blot analysis using ab243874 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (50 µg).
Lane 2: MOLT-4 whole cell lysate (50 µg).
Lane 3: K-562 whole cell lysate (50 µg).
Lane 4: SK whole cell lysate (50 µg).
Lane 5: HeLa whole cell lysate (50 µg).
Lane 6: HEK-293Y whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3 zeta using ab243874 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)

Formalin-fixed, paraffin-embedded human metastatic lymph node tissue stained for CD3 zeta using ab243874 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)

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