Anti-PI-9 antibody (ab233443)
Key features and details
- Rabbit polyclonal to PI-9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-PI-9 antibody
See all PI-9 primary antibodies -
Description
Rabbit polyclonal to PI-9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human PI-9 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P50453 -
Positive control
- IHC-P: Human stomach, cerebrum, prostate cancer, kidney, prostate and bile duct cancer tissues. WB: Recombinant human PI-9 protein; Human serum; Mouse placenta lysate; K562 cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab233443 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI-9 antibody (ab233443)
Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Western blot - Anti-PI-9 antibody (ab233443)Anti-PI-9 antibody (ab233443) at 2 µg/ml + Mouse placenta lysate
Predicted band size: 42 kDa
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Western blot - Anti-PI-9 antibody (ab233443)Anti-PI-9 antibody (ab233443) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 42 kDa
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Western blot - Anti-PI-9 antibody (ab233443)Anti-PI-9 antibody (ab233443) at 3 µg/ml + Human serum
Predicted band size: 42 kDa
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Western blot - Anti-PI-9 antibody (ab233443)Anti-PI-9 antibody (ab233443) at 3 µg/ml + Recombinant human PI-9 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 42 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-PI-9 antibody (ab233443)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PI-9 using ab233443 at 10 µg/ml in ICC/IF. FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody was used as the secondary antibody at 1 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI-9 antibody (ab233443)Formalin-fixed, paraffin-embedded human prostate tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI-9 antibody (ab233443)Formalin-fixed, paraffin-embedded human kidney tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI-9 antibody (ab233443)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI-9 antibody (ab233443)Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI-9 antibody (ab233443)Formalin-fixed, paraffin-embedded human stomach tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
