Human CD276 ELISA Kit (ab221831)
Key features and details
- Sensitivity: 178 pg/ml
- Range: 578 pg/ml - 37000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD276 ELISA Kit
See all CD276 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 2.9% Inter-assay Sample n Mean SD CV% Overall 5 9.9% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
178 pg/ml -
Range
578 pg/ml - 37000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 94 83% - 101% Urine 89 88% - 91% Serum 102 100% - 104% Hep Plasma 87 85% - 89% EDTA Plasma 91 87% - 94% Cit plasma 94 90% - 100% -
Assay time
2h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human CD276 ELISA Kit (ab221831) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD276 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD276 with 178 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD276 (B7-H3) is a glycoprotein member of the B7 family, Ig superfamily of proteins. CD276 is involved in the regulation of T-cell-mediated immune response and is expressed by a variety of cell types.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD276 Capture Antibody 1 x 600µl 10X Human CD276 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CD276 Lyophilized Recombinant Protein 1 x 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. -
Tissue specificity
Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- 4Ig B7 H3
- 4Ig-B7-H3
- AU016588
see all -
Database links
- Entrez Gene: 80381 Human
- Omim: 605715 Human
- SwissProt: Q5ZPR3 Human
- Unigene: 77873 Human
- Unigene: 744915 Human
Images
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Concentrations of CD276 in human serum samples
Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD276 concentration was determined to be 31.5 ng/mL with a range of 18.7-53.6 ng/mL.
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Concentrations of CD276 in human serum and plasma samplesInterpolated concentrations of CD276 in human serum and plasma samples. The concentrations of CD276 were measured in duplicates, interpolated from the CD276 standard curves and corrected for sample dilution. Undiluted samples: serum 50%, plasma (EDTA) 25%, plasma (citrate) 25%, plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD276 concentration was determined to be 31.7 ng/mL in serum, 25.0 ng/mL in plasma (EDTA), 25.1 ng/mL in plasma (citrate) and 28.5 ng/mL in plasma (heparin).
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Human CD276 standard curveExample of human CD276 standard curve in Sample Diluent NS.
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Sandwich ELISA - Human CD276 ELISA Kit (ab221831)To learn more about the advantages of recombinant antibodies see here.
