Mouse DKK1 Matched Antibody Pair Kit (ab212067)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 20 pg/ml - 1250 pg/ml
Overview
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Product name
Mouse DKK1 Matched Antibody Pair Kit
See all DKK1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
3.88 pg/ml -
Range
20 pg/ml - 1250 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse DKK1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse DKK1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab197746. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse DKK1 Lyophilized Protein 2 vials 1 vial Mouse DKK1 Capture Antibody 2 x 50µg 1 x 50µg Mouse DKK1 Detector Antibody 2 x 12.5µg 1 x 12.5µg -
Research areas
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Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
Tissue specificity
Placenta. -
Sequence similarities
Belongs to the dickkopf family. -
Domain
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Dickkopf 1
- Dickkopf 1 homolog
- Dickkopf 1 like
see all -
Database links
- Entrez Gene: 13380 Mouse
- SwissProt: O54908 Mouse
- Unigene: 214717 Mouse
Images
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Mouse DKK1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse DKK1 Matched Antibody Pair Kit (ab212067)To learn more about the advantages of recombinant antibodies see here.
