Mouse DKK1 ELISA Kit (ab197746)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.66 pg/ml
- Range: 39.1 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse DKK1 ELISA Kit
See all DKK1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 5.2% Inter-assay Sample n Mean SD CV% Overall 3 5.26% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6.66 pg/ml -
Range
39.1 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 114 109% - 120% Cell culture media 99 93% - 105% Cit plasma 112 99% - 121% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse DKK1 ELISA Kit (ab197746) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKK1 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse DKK1 with 6.66 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Dickkopf-related proteins are secreted proteins with two cysteine-rich domains. They are important in early vertebrate development due to their antagonistic relationship to Wnt signaling, which is involved in embryonic development, limb development, somitogenesis, and eye formation. In adults Dickkopf-related proteins are important to bone formation and bone related diseases, as well as certain cancers and Alzheimer disease. Dickkopf-related protein 1 (DKK1) is one of two secreted inhibitors of canonical Wnt signaling. DKK1 decreases Wnt signaling by inhibiting the interaction between LRP5/6 and Wnt-frizzled complexes. Elevated DKK1 levels are associated with the presence of osteolytic bone lesions in multiple myeloma patients, and may also contribute to the osteopenia and hypercalcemia often associated with myeloma. The inhibition of DKK1 has shown the reversal of the bone deteriorating patterns associated with osteoarthritis.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse DKK1 Capture Antibody 1 x 600µl 10X Mouse DKK1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Mouse DKK1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
DKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. -
Alternative names
- Dickkopf 1
- Dickkopf homolog 1
- Dickkopf related protein 1
see all -
Database links
- Entrez Gene: 13380 Mouse
- SwissProt: O54908 Mouse
Images
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Other - Mouse DKK1 ELISA Kit (ab197746)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse DKK1 standard curve comparison data.Standard curve comparison between mouse DKK1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 3-fold increase in sensitivity.
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Example of DKK1 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of mouse Serum and mouse plasma within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Titration of protein spiked cell culture media within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Sandwich ELISA - Mouse DKK1 ELISA Kit (ab197746)To learn more about the advantages of recombinant antibodies see here.
