Mouse SLAM ELISA Kit (ab267587)
Key features and details
- Sensitivity: 0.06 ng/ml
- Range: 0.049 ng/ml - 12 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse SLAM ELISA Kit
See all SLAM / CD150 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.06 ng/ml -
Range
0.049 ng/ml - 12 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 84.38 73% - 106% Plasma 89.09 80% - 100% Cell culture media 129 116% - 138% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse SLAM ELISA Kit is designed for the quantitative determination of SLAM in cell culture supernatants, plasma and serum samples.
Δ Note: Collect plasma using citrate and heparin as an anticoagulant. EDTA is not recommended as an anticoagulant for use in this assay due to its chelating properties.
This assay employs an antibody specific for mouse SLAM coated on a 96-well plate. Standards and samples are pipetted into the wells and SLAM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SLAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SLAM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Mouse SLAM coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse SLAM Detection Antibody 2 vials Mouse SLAM Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. -
Tissue specificity
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues. -
Post-translational
modificationsPhosphorylated by FYN. -
Cellular localization
Cell membrane. Present on the surface of B-cells and T-cells. - Information by UniProt
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Alternative names
- 4933415F16
- AA177906
- CD 150
see all -
Database links
- Entrez Gene: 27218 Mouse
- SwissProt: Q9QUM4 Mouse
- Unigene: 103648 Mouse
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.