Anti-CD276 antibody (ab226256)
Key features and details
- Rabbit polyclonal to CD276
- Suitable for: ICC, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CD276 antibody
See all CD276 primary antibodies -
Description
Rabbit polyclonal to CD276 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide within Human CD276 aa 484-534. The exact sequence is proprietary. (NP_001019907.1).
Database link: Q5ZPR3 -
Positive control
- IP: HeLa whole cell lysate (ab150035). IHC-P: Human prostate carconoma tissue ICC: MCF7 cells; CD276 transfected HEK-293 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226256 was affinity purified using an epitope specific to CD276 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody (ab226256)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for CD276 using ab226256 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining. Counterstain: Hematoxylin.
-
Immunoprecipitation - Anti-CD276 antibody (ab226256)CD276 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab226256 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-CD276 antibody at 1 µg/ml.
Lane 1: ab226256 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
-
Immunocytochemistry - Anti-CD276 antibody (ab226256)MCF7 (human breast adenocarcinoma cell line) (left panel) and CD276 transfected HEK-293 (human epithelial cell line from embryonic kidney) (right panel) cells stained for CD276 using ab226256 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
