Anti-RAGEF2 antibody (ab86225)
Key features and details
- Rabbit polyclonal to RAGEF2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAGEF2 antibody -
Description
Rabbit polyclonal to RAGEF2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 625 and 675 of human RAGEF2 (NP_057424.2)
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAGEF2 antibody (ab86225)All lanes : Anti-RAGEF2 antibody (ab86225) at 0.04 µg
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 179 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunoprecipitation - Anti-RAGEF2 antibody (ab86225)Detection of human RAGEF2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86225 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.