Anti-p53 (acetyl K386) antibody (ab52172)
Key features and details
- Rabbit polyclonal to p53 (acetyl K386)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-p53 (acetyl K386) antibody
See all p53 primary antibodies -
Description
Rabbit polyclonal to p53 (acetyl K386) -
Host species
Rabbit -
Specificity
ab52172 detects endogenous levels of p53 only when acetylated at lysine 386. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
-
Immunogen
Synthetic peptide corresponding to Human p53 aa 344-393 (acetyl K386). Synthetic acetylated peptide derived from human p53 around the acetylation site of lysine 386 (M-F-KA-T-E).
Database link: P04637
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab52172 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (acetyl K386) antibody (ab52172)ab52172 at 1/50 dilution staining P53 (acetyl K386) in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence (right image) of the immunising peptide.
-
Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K386) antibody (ab52172)ICC/IF image of ab52172 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52172, 1µg/ml) overnight at +4°C. The secondary antibody (green)was Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
