Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free (ab248440)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7321] to SERCA1 ATPase - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free
    See all SERCA1 ATPase primary antibodies

  • Description

    Rabbit monoclonal [EPR7321] to SERCA1 ATPase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248440 is the carrier-free version of ab133275 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248440 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free (ab248440)
    Western blot - Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free (ab248440)
    Anti-SERCA1 ATPase antibody [EPR7321] (ab133275) at 1/10000 dilution + Fetal muscle tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133275, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 180 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free (ab248440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free (ab248440)
    This data was developed using ab133275, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labelling SERCA1 ATPase using ab133275, at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free (ab248440)
    OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free (ab248440)
    This data was developed using ab133275, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free (ab248440)
    Anti-SERCA1 ATPase antibody [EPR7321] - BSA and Azide free (ab248440)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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