Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20149-224] to Transferrin - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free
    See all Transferrin primary antibodies

  • Description

    Rabbit monoclonal [EPR20149-224] to Transferrin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human breast, kidney, testis and liver tissue lysates; Human plasma. IHC-P: Human liver and testis tissue. ICC: HepG2 cells. Flow cyt: HepG2 cells. IP: Human liver tissue lysate.

  • General notes

    ab277640 is the carrier-free version of ab277635. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277640 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)

    This data was developed using ab277635, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Transferrin with ab277635 at 1/100 (5.04 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human liver. The section was incubated with ab277635 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    Immunocytochemistry - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)

    This data was developed using ab277635, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labelling Transferrin with ab277635 at 1/100 (5.04 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluo® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    Flow Cytometry - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)

    This data was developed using ab277635, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Transferrin with ab277635 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    Immunoprecipitation - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)

    This data was developed using ab277635, the same antibody clone in a different buffer formulation.

    Transferrin was immunoprecipitated from 0.35 mg Human liver tissue lysate with ab277635 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277635 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human liver tissue lysate 10 ug

    Lane 2: ab277635 IP in Human liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab277635 in Human liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    Western blot - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    All lanes : Anti-Transferrin antibody [EPR20149-224] (ab277635) at 1/1000 dilution

    Lane 1 : Human plasma at 20 µl
    Lane 2 : Human breast tissue lysate at 20 µg
    Lane 3 : Human kidney tissue lysate at 20 µg
    Lane 4 : Human testis tissue lysate at 20 µg
    Lane 5 : Human liver tissue lysate at 20 µg

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
    Lanes 2-5 : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa



    This data was developed using ab277635, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1-4: 3.25 seconds.

    Lane 5 15 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)

    This data was developed using ab277635, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Transferrin with ab277635 at 1/100 (5.04 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human testis. The section was incubated with ab277635 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    Western blot - Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    Anti-Transferrin antibody [EPR20149-224] (ab277635) at 1/1000 dilution + HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa



    This data was developed using ab277635, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: This blot was developed using a higher sensitivity ECL substrate.

     

    3 minutes

    Exposure time: 

  • Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)
    Anti-Transferrin antibody [EPR20149-224] - BSA and Azide free (ab277640)

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