Anti-Tissue kallikrein antibody (ab28289)
Key features and details
- Rabbit polyclonal to Tissue kallikrein
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tissue kallikrein antibody
See all Tissue kallikrein primary antibodies -
Description
Rabbit polyclonal to Tissue kallikrein -
Host species
Rabbit -
Specificity
ab28289 recognises the Kallikrein loop of Tissue kallikrein. It does not react with the other human Kallikreins. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to Human Tissue kallikrein. Corresponding to the the Kallikrein loop area of Tissue kallikrein.
(Peptide available asab41419) -
General notes
Protein previously labeled as Kallikrein 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tissue kallikrein antibody (ab28289)All lanes : Anti-Tissue kallikrein antibody (ab28289) at 1/1000 dilution
Lane 1 : Kalikrein 1 at 0.05 µg
Lane 2 : Kalikrein 1 at 0.01 µg
Lane 3 : Kalikrein 1 at 0.001 µg
Predicted band size: 28 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue kallikrein antibody (ab28289)IHC image of ab28289 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28289, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
