Anti-HIV1 p24 antibody [39/5.4A] (ab9071)
![Anti-HIV1 p24 antibody [39/5.4A] (ab9071)](https://www.abcam.com/ps/products/9/ab9071/Images/ab9071-320025-anti-hiv1-p24-antibody-39-54a-western-blot.jpg "Anti-HIV1 p24 antibody [39/5.4A] (ab9071)")
Key features and details
- Mouse monoclonal [39/5.4A] to HIV1 p24
- Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF, Sandwich ELISA
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 p24 antibody [39/5.4A]
See all HIV1 p24 primary antibodies -
Description
Mouse monoclonal [39/5.4A] to HIV1 p24 -
Host species
Mouse -
Specificity
Human Immunodeficiency Virus Type 1(HIV 1) p24 protein. No detectable reaction has been observed with HIV-2 or SIV (Simian Immunodeficiency Virus) viral lysates by ELISA or Western blot. -
Tested applications
Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF, Sandwich ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Tissue, cells or virus corresponding to HIV1 p24. ab9071 raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate.
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General notes
ab9071 is used to detect HIV-1 core protein in virus infected cells, cell lysates, culture fluid, serum or plasma.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from serum-free culture supernatant. -
Primary antibody notes
The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. Useful as solid phase for capture of p24 in an antigen ELISA format. -
Clonality
Monoclonal -
Clone number
39/5.4A -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071) Saito H. et al PLoS One. 2017 Sep 5;12(9):e0182434. doi: 10.1371/journal.pone.0182434. eCollection 2017. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunoblot analysis of viral proteins in HEK293-derived cells transiently transfected with the proviral plasmid, pNL4-3. EV or POM121C (614–987) cells were transfected with 1.0 μg of pNL4-3. The cells were harvested 48 h post-transfection and lysates were subjected to immnoblot analyses with anti-HIV-1 p24 (top panel; the upper arrow indicated precursor Gag [PrGag], the lower arrow indicated CA), anti-HA (middle panel) and anti-CYPA (bottom panel). One representative set of results from three independent experiments is shown.
Whole-cell lysates were prepared as follows: cells were washed twice with phosphate-buffered saline (PBS) (-), suspended in PBS(-) (500 μl per 1 × 107 cells) and mixed with an equal volume of 2 × sample buffer (4% sodium dodecyl sulfate, 125 mM Tris-HCl, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol, and 0.002% bromphenol blue). Proteins were solubilized by heating for 5 min at 95°C. Samples were subjected to SDS-PAGE, transferred to PVDF membranes, and reacted with mouse monoclonal antibody to HIV-1 p24 (#ab9071, Abcam, Inc., Cambridge, MA), or rabbit polyclonal antibody to CYPA (#BML-SA296, Enzo Life Sciences, Inc., Farmingdale, NY). Membranes were then incubated with horseradish peroxidase-conjugated secondary antibody (#NA934 for anti-rabbit IgG, #NA931 for ant-mouse IgG, #NA933 for anti-human IgG, Amersham Biosciences, Piscataway, NJ), and proteins were visualized by Western Lightning Plus-ECL (PerkinElmer, Waltham, MA) or enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL).
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![Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)](https://www.abcam.com/ps/products/9/ab9071/Images/ab9071-16745-anti-hiv1-p24-antibody-39-54a-western-blot.jpg)
Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071) This image is courtesy of an anonymous AbreviewAll lanes : Anti-HIV1 p24 antibody [39/5.4A] (ab9071) at 1/2000 dilution
Lane 1 : Mock transfected 293T cells - cell lysate
Lane 2 : 293T cells transfected with HIV DNA - cell lysate
Secondary
All lanes : HRP conjugated goat anti-mouse IgG
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 24,25,41,55 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
