Anti-TDP43 antibody (ab225710)
Key features and details
- Rabbit polyclonal to TDP43
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TDP43 antibody
See all TDP43 primary antibodies -
Description
Rabbit polyclonal to TDP43 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human TDP43 aa 150-200. The exact sequence is proprietary. NP_031401.1
Database link: Q13148 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human non-small cell lung cancer tissue; mouse renal cell carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody (ab225710)
Formalin fixed, paraffin embedded mouse renal cell carcinoma tissue stained for TDP43 with ab225710 (1/200 dilution) in immunohistochemical analysis. DAB detection.
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Western blot - Anti-TDP43 antibody (ab225710)All lanes : Anti-TDP43 antibody (ab225710) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg/ml
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg/ml
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg/ml
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg/ml
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-TDP43 antibody (ab225710)Whole cell lysate from HeLa (human epithelial cell line from cervix adenocarcinoma) cells (1 mg for IP, 20% of IP loaded). ab225710 used for for WB at 0.4 µg/mg and IP at 6 µg/mg lysate.
Lane 1: ab225710 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody (ab225710)Formalin fixed, paraffin embedded human non-small cell lung cancer tissue stained for TDP43 with ab225710 (1/1000 dilution) in immunohistochemical analysis. DAB detection.
