Recombinant HIV1 Protease protein (ab215523)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
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Product name
Recombinant HIV1 Protease protein
See all HIV1 Protease proteins and peptides -
Biological activity
Reaction buffer: 20 mM Tris-HCl (pH 6.8), 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 10% Glycerol
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Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Sequence
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGI GGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF -
Predicted molecular weight
11 kDa -
Amino acids
501 to 599 -
Additional sequence information
HIV1
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Preparation and Storage
- HIV-1 protease
Images
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Functional Studies - Recombinant HIV1 Protease protein (ab215523)
ELISA of ab215523 using an anti-HIV protease antibody. Purified protease was spotted on wells.
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SDS-PAGE - Recombinant HIV1 Protease protein (ab215523)SDS-PAGE analysis of ab215523.
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Dot Blot - Recombinant HIV1 Protease protein (ab215523)Dot of ab215523. A anti-HIV-1 protease antibody was used at 1/2000 dilution, followed by a goat anti-rabbit IgG antibody conjugated with HRP secondary antibody at 1/5000 dilution.
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SDS-PAGE - Recombinant HIV1 Protease protein (ab215523)SDS-PAGE analysis of proteolytic processing of HIV-1 Gag p55 proprotein by ab215523 in vitro. Recombinant Gag p55 (1 μg) was used as the substrate in a 20 μl reaction volume. The reaction was carried by incubating at 37℃ for 3 h and stopped by adding SDS-PAGE sample buffer. 1; no protease, 2: 0.16 pg. 3; 1.6 pg. 4; 16 pg 5; 0.16 μg . 6; 1.6 μg ab215523. Note that two degradation bands are observed in the preparation of p55 substrate. In lane 4, p25 band is visible and in lane 5, p13 band is visible.
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Western blot - Recombinant HIV1 Protease protein (ab215523)All lanes : Anti HIV1 protease at 1/2000 dilution
Lane 1 :Recombinant HIV1 Protease protein (ab215523) at 0.001 µg
Lane 2 : Extract of MT4 cells
Lane 3 : Extract of MT4 cells infected with HIV-1 (LAI strain)
Predicted band size: 10.8 kDaab215523 as a control antigen for Western blot analysis.