Anti-SART3 antibody (ab176822)
Key features and details
- Rabbit polyclonal to SART3
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SART3 antibody
See all SART3 primary antibodies -
Description
Rabbit polyclonal to SART3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Orangutan
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Immunogen
Synthetic peptide within Human SART3 aa 600-650. The exact sequence is proprietary.
Sequence:Q RKRARAEKKA LKKKKKIRGP EKRGADEDDE KEWGDDEEEQ PSKRRRVENS
Database link: Q15020 -
Positive control
- HeLa whole cell lysate (ab150035); Human ovarian and colon carcinoma tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176822 was affinity purified using an epitope specific to SART3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SART3 antibody (ab176822)All lanes : Anti-SART3 antibody (ab176822) at 0.02 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 110 kDa
Exposure time: 3 seconds
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Immunoprecipitation - Anti-SART3 antibody (ab176822)Detection of SART3 by Western Blot of Immunoprecipitate.
abX176822 at 0.04µg/ml labeling SART3 in HeLa whole cell lysate immunoprecipitated using ab176822 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure times of 3
seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SART3 antibody (ab176822)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling SART3 with ab176822 at 1 µg/ml.
Detection: DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-SART3 antibody (ab176822)Immunohistofluorescent analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SART3 with ab176822 at 0.5 µg/ml.
Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100.
