Anti-SART3 antibody (ab224810)
Key features and details
- Rabbit polyclonal to SART3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SART3 antibody
See all SART3 primary antibodies -
Description
Rabbit polyclonal to SART3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human SART3 aa 913-963. The exact sequence is proprietary. (NP_055521.1).
Database link: Q15020 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab224810 was affinity purified using an epitope specific to SART3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SART3 antibody (ab224810)All lanes : Anti-SART3 antibody (ab224810) at 0.02 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 110 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-SART3 antibody (ab224810)SART3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224810 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224810 at 0.04 µg/ml.
Lane 1: ab224810 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
