Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [DB9] to TDP43 - BSA and Azide free
  • Suitable for: WB, IHC-P, ELISA
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TDP43 antibody [DB9] - BSA and Azide free
    See all TDP43 primary antibodies

  • Description

    Mouse monoclonal [DB9] to TDP43 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human TDP43 aa 200 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q13148

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, Jurkat, K562, HEK-293T, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Human cerebrum and colon tissue; mouse cerebrum tissue; rat cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255922 is the carrier-free version of ab254166. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TDP43 with ab254166 at 4.67µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human colon is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254166).

  • Western blot - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)
    Western blot - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)
    All lanes : Anti-TDP43 antibody [DB9] (ab254166) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
    Lane 4 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
    Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate at 10 µg
    Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
    Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
    Lane 8 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-4: 103 seconds;
    Lanes 5-8: 48 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254166).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TDP43 with ab254166 at 23.35µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on rat cerebrum is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254166).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TDP43 with ab254166 at 23.35µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on mouse cerebrum is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254166).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TDP43 with ab254166 at 23.35µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human cerebrum is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254166).

  • ELISA - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)
    ELISA - Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)

    TDP43 = 5000 ng/mL.

    ab254166 used at 0 - 10,000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG （H+L）secondary antibody was used at 1/1000 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254166).

  • Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)
    Anti-TDP43 antibody [DB9] - BSA and Azide free (ab255922)

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