Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free (ab247212)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free
    See all CDK1+CDK2+CDK3+CDK5 primary antibodies

  • Description

    Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247212 is the carrier-free version of ab32030 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247212 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free (ab247212)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free (ab247212)
    All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/10000 dilution

    Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
    Lane 2 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
    Lane 3 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking and dilution buffer: 5% NFDM/TBST.

    The endogenous CDK is detected at higher exposure.

    We are not sure the bands around 35kDa. Perhaps they are degradation products.

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free (ab247212)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free (ab247212)
    All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/2000 dilution

    Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
    Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
    Lane 3 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa


    Exposure time: 15 seconds


    This data was developed using ab32030, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free (ab247212)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free (ab247212)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/5000 dilution + HeLa cell lysate

    Predicted band size: 34 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    This data was developed using ab32030, the same antibody clone in a different buffer formulation.

     

  • Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free (ab247212)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] - BSA and Azide free (ab247212)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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