Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µg 314 937 ₸ 10 µg 107 212 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [DB9] to TDP43
Suitable for: WB, IHC-P, ELISA
Reacts with: Mouse, Rat, Human

Product name

Anti-TDP43 antibody [DB9]
See all TDP43 primary antibodies
Description

Mouse monoclonal [DB9] to TDP43
Host species

Mouse

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment within Human TDP43 aa 200 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13148

Run BLAST with Run BLAST with
Positive control
- WB: HeLa, Jurkat, K562, HEK-293T, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Human cerebrum and colon tissue; mouse cerebrum tissue; rat cerebrum tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

DB9
Isotype

IgG1
Research areas

Western blot - Anti-TDP43 antibody [DB9] (ab254166)

All lanes : Anti-TDP43 antibody [DB9] (ab254166) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 4 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate at 10 µg
Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 8 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

Predicted band size: 44 kDa
Observed band size: 44 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1-4: 103 seconds;
Lanes 5-8: 48 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TDP43 with ab254166 at 4.67µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human colon is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TDP43 with ab254166 at 23.35µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on rat cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TDP43 with ab254166 at 23.25µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on mouse cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TDP43 with ab254166 at 4.67µg/ml followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
ELISA - Anti-TDP43 antibody [DB9] (ab254166)

TDP43 = 5000 ng/mL.

ab254166 used at 0 - 10,000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG （H+L）secondary antibody was used at 1000ng/ml
Anti-TDP43 antibody [DB9] (ab254166)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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