Anti-HIV1 tat antibody - ChIP Grade (ab43014)
Key features and details
- Rabbit polyclonal to HIV1 tat - ChIP Grade
- Suitable for: WB
- Isotype: IgG
Overview
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Product name
Anti-HIV1 tat antibody - ChIP Grade
See all HIV1 tat primary antibodies -
Description
Rabbit polyclonal to HIV1 tat - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant full length protein expressed in E.coli.
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Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HIV1 tat antibody - ChIP Grade (ab43014) This image is courtesy of an anonymous AbreviewAll lanes : Anti-HIV1 tat antibody - ChIP Grade (ab43014) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate 0 hours after infection
Lane 2 : Jurkat whole cell lysate 2 hours after infection
Lane 3 : Jurkat whole cell lysate 4 hours after infection
Lane 4 : Jurkat whole cell lysate 6 hours after infection
Lane 5 : Jurkat whole cell lysate 8 hours after infection
Lane 6 : Jurkat whole cell lysate 10 hours after infection
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 14 kDa why is the actual band size different from the predicted?
Jurkat cells were pNL4.3 infected and samples taken at 2 hour intervals for blotting.
