Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)
![Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)](https://www.abcam.com/ps/products/259/ab259802/Images/ab259802-1-anti-hladpb1-antibody-epr239471-immunohistochemistry-human-tonsil.jpg "Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23947-1] to HLA-DPB1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-HLA-DPB1 antibody [EPR23947-1]
See all HLA-DPB1 primary antibodies -
Description
Rabbit monoclonal [EPR23947-1] to HLA-DPB1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB HumanRecombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: daudi whole cell lysate; Human tonsil and spleen tissue lysates; HEK-293T transfected with HLA-DPB1 expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: Human tonsil, liver and epityphlon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23947-1 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling HLA-DPB1 with ab259802 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with ab259802 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)](https://www.abcam.com/ps/products/259/ab259802/Images/ab259802-448248-anti-hladpb1-antibody-epr239471-western-blot-lymphoma.jpg)
Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)All lanes : Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802) at 1/1000 dilution
Lane 1 : Daudi (Human Burkitt’s lymphoma lymphoblast), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lane 3 : Human tonsil tissue lysate
Lane 4 : Human spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HeLa.
The expression profile/molecular weight observed is consistent with what has been described in the literature. Band detected around 54 kDa could be the dimeric form (PMID: 20959457).
This blot was developed using a higher sensitivity ECL substrate.
Exposure times: Lane 1: 81 seconds.
Lane 2-4: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)](https://www.abcam.com/ps/products/259/ab259802/Images/ab259802-2-anti-hladpb1-antibody-epr239471-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HLA-DPB1 with ab259802 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on Kupffer cells in human liver. The section was incubated with ab259802 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)](https://www.abcam.com/ps/products/259/ab259802/Images/ab259802-448249-anti-hladpb1-antibody-epr239471-western-blot-class.jpg)
Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)All lanes : Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802) at 1/1000 dilution
Lane 1 : His-tagged human HLA class II histocompatibility antigen, DRB1 beta chain recombinant protein, 35 ng
Lane 2 : HEK-293T transfected with HLA-DPB1 expression vector containi a myc-His-tag®, whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 29 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)](https://www.abcam.com/ps/products/259/ab259802/Images/ab259802-3-anti-hladpb1-antibody-epr239471-immunohistochemistry-human-epityphlon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)Immunohistochemical analysis of paraffin-embedded human epityphlon tissue labeling HLA-DPB1 with ab259802 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human epityphlon. The section was incubated with ab259802 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)](https://www.abcam.com/ps/products/259/ab259802/Images/ab259802-448571-benefitsofrecombinantantibodies.png)
Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802)