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Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [IIH11] to SERCA1 ATPase - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free
    See all SERCA1 ATPase primary antibodies

  • Description

    Mouse monoclonal [IIH11] to SERCA1 ATPase - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat skeletal muscle tissue lysate. IHC-P: Mouse and rat skeletal muscle tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255961 is the carrier-free version of ab2818. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
    Western blot - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
    All lanes : Anti-SERCA1 ATPase antibody [IIH11] (ab2818) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle tissue lysate
    Lane 2 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L), at 1/10000 dilution

    Predicted band size: 110 kDa


    Exposure time: 48 seconds


    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab2818).

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab2818).

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117µg/ml, followed by ready to use secondary. Cytoplasmic staining on rat skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab2818).

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117µg/ml, followed by ready to use secondary. Cytoplasmic staining on mouse skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

  • Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
    Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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