Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10364] to P Glycoprotein - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free
    See all P Glycoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPR10364] to P Glycoprotein - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands ab168337 detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150 – 300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Human P Glycoprotein.

  • Positive control

    • HeLa, HepG2, 293T, C6 and Human fetal brain and Mouse brain lysates, Human kidney & liver tissue

  • General notes

    The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

    ab271921 is the carrier-free version of ab168337. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)
    Western blot - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: P glycoprotein knockout HAP1 cell lysate (20 µg) 
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)


    Lanes 1 - 4: Merged signal (red and green). Green - ab168337 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab168337 was shown to recognize P glycoprotein when P glycoprotein knockout samples were used, along with additional cross-reactive bands. Wild-type and P glycoprotein knockout samples were subjected to SDS-PAGE. ab168337 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168337).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab168337 at 1/100 dilution (14 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168337).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling P Glycoprotein with unpurified ab168337 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168337).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling P Glycoprotein with unpurified ab168337 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168337).
  • Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)
    Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)

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